簡志青教授
工作執掌
授課科目：
普通化學
生技與生醫概論
工程專題討論（二）
學歷
台北醫學院醫事技術學系理學士
美國康乃迪克大學微生物學碩士
美國康乃迪克大學微生物學博士
經歷
美國麻州海洋生物實驗室,1995年微生物多樣性學員
麻省理工學院博士後研究
密蘇里大學化工系 博士後研究 ( 1998/09 ~ 1999/04)
加州理工學院助理科學家
工研院生醫工程中心分子生醫技術組研究員
工研院生醫工程中心分子生醫技術組蛋白體技術部副經理
研究專長
1.工業與應用微生物
2.微生物多樣性與環境微生物
3.生物降解與環境毒理學
4.環境生技與分子生物學
期刊論文
(1) Horng, Y-T., Jeng, W-Y., Chen,Y-Y., Liu, C-H., Dou, H-Y., Lee, J-J., Chang, K-C., Chien, C-C., Soo, P-C. (2015) Molecular analysis of codon 548 in the rpoB gene involved in Mycobacterium tuberculosis resistance to rifampicin. Antimicrob. Agents Chemother. (Accepted) [SCI]
(2) Chen, L.-C., Chueh, T.-C., Tuan, Y.-F., Chen, C.-C., Chien, C.-C., Lee, H.-Y., Chen, S.-C.* (2015) Activation of MAPK pathways and downstream transcription factors in 2-aminobiphenyl-induced apoptosis. Environ Toxicol (In press) [SCI]
(3) Sheu, Y.T., Chen, S.C., Chien, C.C., Chen, C.C., Kao, C.M.* (2015) Application of a long-lasting colloidal substrate with pH and hydrogen sulfide control capabilities to remediate TCE-contaminated groundwater. J Hazard Mater 284: 222-232. [SCI]
(4) Chien C-C* , Kao C-M, Chen D-Y, Chen SC, Chen C-C. (2014) Biotransformation of trinitrotoluene (TNT) by Pseudomonas spp. isolated from a TNT-contaminated environment. Environ Toxicol Chem 33 (5):1059-1063. [SCI]
(5) Chien C-C *, Wang L-J, Lin W-R (2014) Polyhydroxybutyrate accumulation by a cadmium-resistant strain of Cupriavidus taiwanensis. J Taiwan Ins Chem Eng 45 (4): 1164-1169. [SCI]
(6) Chien, C.-C.*, Lin, B.-C., Wu, C.-H. (2013) Biofilm formation and heavy metal resistance by an environmental Pseudomonas sp. Biochem Eng J 78:132-137. [SCI]
(7) Chen, W.-C., Yu, W.-J., Chang, C.-C., Chang, J.-S., Huang, S.-H., Chang, C.-H., Chen, S.-Y., Chien, C.-C., Yao, C.-L., Chen, W-M., Wei, Y.-H.* (2013) Enhancing production of prodigiosin from Serratia marcescens C3 by statistical experimental design and porous carrier addition strategy. Biochem Eng J 78:93-100. [SCI]
(8) Horng, Y.-T., Chien, C.-C., Huang, C.-T., Wei, Y.-H., Chen, S.-Y., Lan, J.C.-W., Soo, P.-C.* (2013) Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with co-expressed propionate permease (prpP), beta-ketothiolase B (bktB), and propionate-CoA synthase (prpE) in Escherichia coli. Biochem Eng J 78:73-79. [SCI]
(9) Chien, C.-C.*, Huang, C.-H., Lin, Y.-W. (2013) Characterization of a heavy metal translocating P-type ATPase gene from an environmental heavy metal resistance Enterobacter sp. isolate. Appl Biochem Biotechnol 169(6): 1837-1846. [SCI]
(10) Chien, C-C.*, Li, H-H., Soo, P-C., Chen, S-Y., Wei, Y-H., Chen, W-M. (2012) Effects of different substrate composition on biosynthesis of polyhydroxybutyrate-co-hydroxyvalerate by recombinant Escherichia coli. Appl Biochem Biotechnol 166(3): 796-804. [SCI]
(11) Chien, C-C.*, Jiang, M-H., Tsai, M-R., Chien, C-C. (2011) Isolation and characterization of an environmental cadmium and tellurite resistant Pseudomonas strain. Environ Toxicol Chem 30(10): 2202-2207. [SCI].
(12) Chu, F.-Y.*, Su, F.-H., Cheng, S.-H., Lin, Y.-S., Li, C.-Y., Chien, C.-C., Lin, Y.-C., Chiang, S.-Y. (2011) Hepatitis B surface antigen confirmatory testing for diagnosis of hepatitis b virus infection in . J Med Virol 83:1514–1521. [SCI]
(13) Horng, Y-T., Chien, C-C., Wei, Y-H., Chen, S-Y., Lan, J.C-W., Sun, Y-M., Soo, P-C.* (2011) Functional cis-expression of phaCAB genes for poly(3-hydroxybutyrate) production by Escherichia coli. Lett Appl Microbiol 52(5):475-483. [SCI]
(14) Chen S.C., Chen C. S., Zhan K-V., Yang K-H., Chien C-C., Shieh B-S., Chen W-M. (2011) Biodegradation of methyl tert-butyl ether (MTBE) by Enterobacter sp. NKNU02. J Hazard Mater 186 (2-3): 1744-1750 [SCI]
(15) Chien, C-C.*, Hong, C-C., Soo, P-C., Wei, Y-H., Chen, S-Y., Cheng, M-L., Sun, Y-M. (2010) Functional expression of phaCAB genes from Cupriavidus taiwanensis strain 184 in Escherichia coli for polyhydroxybutyrate production. Appl Biochem Biotechnol 162(8):2355–2364 [SCI]
(16) Horng, Y-T., Chang, K-C., Chou, T-C., Yu, C-J., Chien, C-C., Wei, Y-H., Soo, P-C.* (2010) Inactivation of dhaD and dhaK abolishes by-product accumulation during 1,3-propanediol production in Klebsiella pneumoniae. J Ind Microbiol Biotechnol 37(7):707–716. [SCI]
(17) Kao, C-M., Chen, CS., Tsa, F-Y., Yang, K-H., Chien, C-C., Liang, S-H., Yang, C-A., Chen, SC.* (2010) Application of real-time PCR, DGGE fingerprinting, and culture-based method to evaluate the effectiveness of intrinsic bioremediation on the control of petroleum-hydrocarbon plume. J Hazard Mater 178(1-3):409-416. [SCI]
(18) Horng, Y-T., Chang, K-C., Chien, C-C., Wei, Y-H., Sun, Y-M., Soo, P-C.* (2010) Enhanced polyhydroxybutyrate (PHB) production via the coexpressed phaCAB and vgb genes controlled by arabinose PBAD promoter in Escherichia coli. Lett Appl Microbiol 50(2): 158-167. [SCI]
(19) Hung, HF., Kuo, YM.*, Chien, CC., Chen, CC. (2010) Use of floating balls for reducing bacterial aerosol emissions from aeration in wastewater treatment processes. J Hazard Mater 175(1-3):866-871. [SCI]
(20) Chien, C-C.*, Han, C-T. (2009) Tellurite resistance and reduction by a Paenibacillus sp. isolated from heavy metal–contaminated sediment. Environ Toxicol Chem 28(8):1627-1632. [SCI]
(21) Chien, C-C.*, Ho, L-Y. (2008) Polyhydroxyalkanoates production from carbohydrates by a genetic recombinant Aeromonas sp. Lett Appl Microbiol 47:587-593. [SCI]
(22) Chien, C-C.*, Tsai, M-L., Chen, C-C., Chang, S-J., Tseng, C-H. (2008) Effects on tyrosinase activity by the extracts of Ganoderma lucidum and related mushrooms. Mycopathologia 166(2):117-120. [SCI]
(23) Wei, Y-H.*, Cheng, C-L., Chien C-C., Wan, H-M. (2008) Enhanced di-rhamnolipid production with an indigenous isolate Pseudomonas aeruginosa J16. Process Biochem 43(7): 769-774. [SCI]
(24) Chien, C-C.* (2008) Taurine serves as sole source of nitrogen for aerobic and anaerobic growth by Klebsiella sp. World J Microbiol Biotechnol 24(6): 895-898. [SCI]
(25) Chien, C-C.*, Kuo, Y-M., Chen, C-C., Hung, C-W., Yeh, C-W., Yeh, W-J. (2008) Microbial diversity of soil bacteria in agricultural field contaminated with heavy metals. J Environ Sci 20(3): 359-363. [SCI]
(26) Su, F-H., Cheng, S-H., Li, C-Y., Chen, J-D., Hsiao, C-Y., Chien, C-C., Yang, Y- C., Hung, H-H., Chu, F-Y. * (2007) Hepatitis B seroprevalence and anamnestic response amongst Taiwanese young adults with full vaccination in infancy, twenty years subsequent to national hepatitis B vaccination. Vaccine 25(47): 8085-8090. [SCI]
(27) Chien, C-C.*, Chen C-C, Choi, M-H., Kung, S-S., Wei, Y-H. (2007) Production of poly-ß-hydroxybutyrate (PHB) by Vibrio spp. isolated from marine environment. J Biotechnol 132(3):259-263. [SCI]
(28) Kung, S-S., Chuang, Y-C., Chen, C-H., Chien, C-C*. (2007) Isolation of polyhydroxyalkanoates (PHAs) producing bacteria using a combination of phenotypic and genotypic approach. Lett Appl Microbiol 44:364-371. [SCI]
(29) Chien, C-C.*, Hung, C-W., Han, C-T. (2007) Removal of cadmium ions during stationary growth phase by an extremely cadmium-resistant strain of Stenotrophomonas sp. Environ Toxicol Chem 26(4):664-668. [SCI]
(30) Chien, C-C.* (2005) Arylsulfonates as sole source of sulfur for Clostridium pasteurianum DSM 12136. J Basic Microbiol 45(4): 274-278. [SCI]
相關著作與專利
專書論著
(1) Chien, C-C. (2008) Polyhydroxyalkanoates: In nature, in the laboratory and in industry. In: "Biotechnology: Research, Technology and Applications." (Editor: Felix W. Richter) Nova Science Publishers, Inc. Hauppauge, NY, . pp. 47-70.
(2) Chien, C-C.(2013) Heavy Metal Niches: Microbes and Their Metabolic Potentials. In: “Applications of Microbial Engineering.” (Editors: Vijai Kumar Gupta, Monika Schmoll, Marcio Antonio Mazutti, Minna Maki, Maria G. Tuohy) CRC Press, Taylor & Francis Group, Boca Raton, FL, USA. (ISBN 978-1-4665-8577-5) pp. 342-366.
專利
(1) 簡志青，蘇伯琦，洪育芝，陳姍玗。「一種使大腸桿菌大量表現丙酸代謝相關基因與合成聚羥基丁酯-羥基戊酯之方法」。中華民國專利申請號：100135702。
(2) 簡志青，蘇伯琦，洪育芝，陳姍玗。「藉由重組大腸桿菌以生產聚羥基丁酸酯(PHB)的方法」。中華民國專利申請號：100103238。
(3) 簡志青，孫一明，康世旭，陳昌傑，何玲儀。「可使微生物利用不同碳源以合成PHA之方法」。中華民國專利I374190，公告日: 101年10月11日。
(4) 康世旭 (Kang, S-H.)，孫一明 (Sun, Y-M.)，簡志青 (Chien, C-C.)，唐世珍 (Tang, S-C.)，陳昌傑 (Chen, C-C.)。Method for production of high purity polyhydroxyalkanoates (PHAs)。US Patent 8053219 B2 (Nov 8, 2011)。